The Potency of β-Sitosterol Isolated From Hydroid Aglaophenia cupressina Lamoureoux as Antimitotic Agent towards Sea Urchin Tripneustes gratilla Linn.
), Sjafaraenan Sjafaraenan(2), E. Johannes(3), M. Litaay(4),
(1) Graduate Student of Department of Biology, Faculty of Mathematics and Natural Sciences
(2) Department of Biology, Faculty of Mathematics and Natural Science Hasanuddin University, Makassar 90245
(3) Department of Biology, Faculty of Mathematics and Natural Science Hasanuddin University, Makassar 90245
(4) Department of Biology, Faculty of Mathematics and Natural Science Hasanuddin University, Makassar 90245
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Abstract
The potency of β-Sitosterol isolated from hydroid Aglaophenia cupressina Lamoureoux as antimitotic agent towards early cleavage of Sea Urchin Tripneustes gratilla Linn had been conducted. The study aimed to determine β-Sitosterol activity isolated compound from hydroid A. cupressina Lamoureoux as antimitotic compound towards early cleavage of zygote of Sea Urchin T. gratilla Linn. Antimitotic treatments were β-Sitosterol (concentration 1 µg/ml, 10 µg/ml and100 µg/ml), positive control vincristine (concentration 0.01 µg/ml, 0.1 µg/ml, and 1 µg/ml) and negative control. A protozoa-free seawater was used in this assay. The results revealed that β-Sitosterol compound had the highest inhibition activity at concentration of 100 μg/ml was 69.22 % with IC-50 on 0.95 µg/ml which could develop as cancer chemotherapy compound.
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