Validation of Reversed-phase High-performance Liquid Chromatography Method for Determination of Alpha Tocopherol in Corn Oil

Abstract

Determination of alpha tocopherol in corn oil can be analyzed by using Reversed-Phase High Performance Liquid Chromatography (RP-HPLC). This research aimed to validate RP-HPLC method for determination of alpha tocopherol in corn oil. The method was based on linearity, precision, accuracy, limit detection (LOD) and limit quantization (LOQ). The Reversed Phase High Performance Liquid Chromatography (RP-HPLC) method includes the determination of the wavelength, the mobile phase composition and the flow rate of mobile phase, then validation of each of the above methods.  The maximum wavelength was found in 290 nm, composition of mobile phase was 100% methanol, flow rate of mobile phase was 1,5 mL/min and retention time was 9,67 min. The researched result indicated that the method of RP-HPLC had a linearity with the correlation coefficient of 0.999, a relative standard deviation (%RSD) for intra and inter-day precision was < 5%, accuracy/recovery was 95.69-100.99 %, detection limit was 0,43 mg/L,  quantization limit was 0,55 mg/L and  alpha tocopherol content  in corn oil was 40.06 ± 0.24 mg/L.