Viability of Entomopathogenic Fungi Metarhizium anisopliae (Metsch) Sorokin in Some Alternative Media and Different Shelf-Life
Lutfi Afifah, Rizky Desriana, Anik Kurniati, Rosalia Maryana
Abstract
Pest control by using chemical pesticides continuously can harm the environment. Therefore it is necessary to look for environmentally friendly technologies, one of which is the use of entomopathogenic fungi such as Metarhizium anisopliae. The alternative media should be easy to obtain and has a good shelf life so the growth of M. anisopliae  should be optimum. This research aimed to obtain alternative media that provide the best growth and development at each different shelf-life. The research method used was an experimental method using a Completely Randomized Design (CRD) Factorial and it was repeated 4 times. The media factors were from PDA, bran, green beans, and maize. The shelf-life factors were at 21 and 42 days old (d-old). The diameter on maize media gave the highest diameter value of 8.57 cm at 42 d-old. Green bean media gave the best spore density results of 2.08×107. Whereas the germination capacity of the media ranged from 63.94% to 94.23%. Germination showed no significant difference at 21 and 42 d-old. Therefore, green beans and maize media were effectively used for propagation of M. anisopliae  as a substitute for synthetic media.
Keywords
Alternative media; conidial spore; germination; M. anisopliae ; shelf-life.
DOI:
http://dx.doi.org/10.20956/ijas.v8i2.2478
Refbacks
There are currently no refbacks.
<div class="statcounter"><a title="shopify analytics" href="http://statcounter.com/shopify/" target="_blank"><img class="statcounter" src="http://c.statcounter.com/10439445/0/0a4c7e05/0/" alt="shopify analytics"></a></div>
IJAS (ISSN Online: 2580-6815 | ISSN Print: 2337-9782) by http://pasca.unhas.ac.id/ojs/index.php/ijas is licensed under a Creative Commons Attribution-ShareyAlike 4.0 International License .
<div class="statcounter"><a title="web counter" href="http://statcounter.com/free-hit-counter/" target="_blank"><img class="statcounter" src="http://c.statcounter.com/10439445/0/0a4c7e05/0/" alt="web counter"></a></div>
View My Stats
International Journal of Agriculture System (IJAS) has been covered by the following indexers: